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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
17/02/2012 |
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Data da última atualização: |
05/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BATISTA, J. S. da S.; HUNGRIA, M. |
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Afiliação: |
JESIANE STEFÂNIA DA SILVA BATISTA, UEL; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Proteomics reveals differential expression of proteins related to a variety of metabolic pathways by genistein-induced Bradyrhizobium japonicum strains. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Proteomics, v. 75, n. 4, p. 1211-1219, Feb. 2012. |
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DOI: |
10.1016/j.jprot.2011.10.032 |
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Idioma: |
Português |
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Conteúdo: |
The rhizobia?legume symbiosis requires a coordinated molecular interaction between the symbionts, initiated by seed and root exudation of several compounds, mainly flavonoids, that trigger the expression of nodulation genes in the bacteria. Since the role of flavonoids seems to be broader than the induction of nodulation genes, we aimed at characterizing genistein-induced proteins of Bradyrhizobium japonicum CPAC 15 (=SEMIA 5079), used in commercial soybean inoculants in Brazil, and of two genetically related strains grown in vitro. Whole-cell proteins were extracted both from induced (1 ?M genistein) and from non-induced cultures of the three strains, and separated by twodimensional electrophoresis. Spot profiles were compared between the two conditions and selected spots were excised and identified by mass spectrometry. Forty-seven proteins were significantly induced by genistein, including several hypothetical proteins, the cytoplasmic flagellar component FliG, periplasmic ABC transporters, a protein related to biosynthesis of exopolysaccharides (ExoN), and proteins involved in redox-state maintenance. Noteworthy was the induction of the PhyR-?EcfG regulon, recently demonstrated to be involved in the symbiotic efficiency of, and general stress response in B. japonicum. Our results confirm that the role of flavonoids, such as genistein, can go far beyond the expression of nodulation-related proteins in B. japonicum. |
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Palavras-Chave: |
Fixação biológica de nitrogênio. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02013naa a2200157 a 4500
001 1916084
005 2017-08-05
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1016/j.jprot.2011.10.032$2DOI
100 1 $aBATISTA, J. S. da S.
245 $aProteomics reveals differential expression of proteins related to a variety of metabolic pathways by genistein-induced Bradyrhizobium japonicum strains.
260 $c2012
520 $aThe rhizobia?legume symbiosis requires a coordinated molecular interaction between the symbionts, initiated by seed and root exudation of several compounds, mainly flavonoids, that trigger the expression of nodulation genes in the bacteria. Since the role of flavonoids seems to be broader than the induction of nodulation genes, we aimed at characterizing genistein-induced proteins of Bradyrhizobium japonicum CPAC 15 (=SEMIA 5079), used in commercial soybean inoculants in Brazil, and of two genetically related strains grown in vitro. Whole-cell proteins were extracted both from induced (1 ?M genistein) and from non-induced cultures of the three strains, and separated by twodimensional electrophoresis. Spot profiles were compared between the two conditions and selected spots were excised and identified by mass spectrometry. Forty-seven proteins were significantly induced by genistein, including several hypothetical proteins, the cytoplasmic flagellar component FliG, periplasmic ABC transporters, a protein related to biosynthesis of exopolysaccharides (ExoN), and proteins involved in redox-state maintenance. Noteworthy was the induction of the PhyR-?EcfG regulon, recently demonstrated to be involved in the symbiotic efficiency of, and general stress response in B. japonicum. Our results confirm that the role of flavonoids, such as genistein, can go far beyond the expression of nodulation-related proteins in B. japonicum.
653 $aFixação biológica de nitrogênio
700 1 $aHUNGRIA, M.
773 $tJournal of Proteomics$gv. 75, n. 4, p. 1211-1219, Feb. 2012.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
07/01/2019 |
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Data da última atualização: |
07/01/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PAREIRA, C. S.; ARRUDA, A. R. R.; ROCHA, S. M. B.; ANTUNES, J. E. L.; ARAUJO, A. S. F. de; HUNGRIA, M. |
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Afiliação: |
UFPI; UFPI; UFPI; UFPI; UFPI; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Diversidade de bactérias isoladas em nódulos de feijão-fava (phaseolus lunatus L.). |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: REUNIÃO NORDESTINA DE CIÊNCIA DO SOLO, 4.; SIMPÓSIO PIAUIENSE DE CIÊNCIA DO SOLO, 1., 2017, Teresina. Uso sustentável do solo e segurança alimentar no nordeste brasileiro. Anais...Teresina(PI): NRNE/SBCS, Embrapa Meio-Norte, UFPI, UESPI, IFPI, 2018. |
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Páginas: |
não paginado. |
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Idioma: |
Português |
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Thesagro: |
Feijão Fava; Leguminosa; Taxonomia. |
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Thesaurus NAL: |
Beans; Taxonomy. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/189879/1/Pareira-RNCS-2017.pdf
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Marc: |
LEADER 00877nam a2200229 a 4500
001 2103194
005 2019-01-07
008 2018 bl uuuu u00u1 u #d
100 1 $aPAREIRA, C. S.
245 $aDiversidade de bactérias isoladas em nódulos de feijão-fava (phaseolus lunatus L.).$h[electronic resource]
260 $aIn: REUNIÃO NORDESTINA DE CIÊNCIA DO SOLO, 4.; SIMPÓSIO PIAUIENSE DE CIÊNCIA DO SOLO, 1., 2017, Teresina. Uso sustentável do solo e segurança alimentar no nordeste brasileiro. Anais...Teresina(PI): NRNE/SBCS, Embrapa Meio-Norte, UFPI, UESPI, IFPI$c2018
300 $anão paginado.
650 $aBeans
650 $aTaxonomy
650 $aFeijão Fava
650 $aLeguminosa
650 $aTaxonomia
700 1 $aARRUDA, A. R. R.
700 1 $aROCHA, S. M. B.
700 1 $aANTUNES, J. E. L.
700 1 $aARAUJO, A. S. F. de
700 1 $aHUNGRIA, M.
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